430 Comparative Analysis of Patients with Birch Pollinosis and Patients with Associated Plant Food Allergy

d 1 and Api g 1. Serum of only one patient revealed the presence of antibodies cross-reacting with the apple, celery and carrot protein with the same molecular weight, which may correspond the main allergens of these foods – Mal d 1, Api g 1 Dau c 1. Additionally sera of 6 persons demonstrated the presence of antibodies reacting with apple protein with the molecular weight 10 kDa which may correspond to the lipid transfer protein (LTP). Among some of the patients, antibodies which have not been identified so far reacted with birch, apple and celery proteins. Conclusions: Although the immunoblotting is an effective method confirming the existences of the cross-reactivity, it still remains the method of verifying and supplementing other diagnostic tests, and a negative result doesn’t exclude the existence of this kind of allergy.